uHPLC Simulation








1^{st} Column


Given Parameters


2^{nd}
Column


Calculated Parameters



Length

ID



Length

ID


cm

mm


cm

mm














Flow rate


Linear Flow

t0


Flow rate


Increased Flow

t0

mL/min

µL/min

cm/min

min


ml/min

µL/min

x Factor

min












Particle Size

Pressure




Particle Size

Pressure



µm

bar

Mpa

Psi


µm

bar

Mpa

Psi












Peak 1


Peak 2



Peak 1


Peak 2


Ret.Time [min]


Ret.Time [min]



Ret.Time [min]


Ret.Time [min]


KValue


KValue



KValue


KValue


Plates/Column


Plates/Column



Plates/Column


Plates/Column


Volume [µL]


Volume [µL]



Volume [µL]


Volume [µL]





Injection Volume

Loading


Concentration


Injection Volume

Loading


Concentration

µL

µg

ng

µg/mL


µL

µg

ng

µg/mL

















Flow cell


Selectivity α



Flow cell


Selectivity α


Path length [mm]


Resolution



Path length [mm]


Resolution


Volume [µL]


Sensitivity

1,00


Volume [µL]


Sensitivity





Sensivity

Resolution

Pressure

Time






Gradient



1^{st} Linear DownScaling




With
same flow rate the gradient in the relationship of the column lengths is
shortened and/or extended


Gradient Column 1



Gradient Column 2


Pressure


Flow Rate (mL/min)



Flow Rate (mL/min)


bar

psi

Time (min)

% B


Time (min)

% B





















































2^{nd} Constant Gradient Volume



Independently
of the column length the same eluent volume is always rinsed through the
column


Gradient Column 1



Gradient Column 2


Pressure


Flow Rate (mL/min)



Flow Rate (mL/min)


bar

psi

Zeit (min)

% B


Zeit (min)

% B
























































Vers.:4.6

© Jürgen MaierRosenkranz


info@jmranalytik.de
