uHPLC Simulation
1st Column		Given Parameters			2nd Column		Calculated Parameters
Length	ID				Length	ID
cm	mm				cm	mm
Flow rate		Linear Flow	t0		Flow rate		Increased Flow	t0
mL/min	µL/min	cm/min	min		ml/min	µL/min	x Factor	min
Particle Size	Pressure				Particle Size	Pressure
µm	bar	Mpa	Psi		µm	bar	Mpa	Psi
Peak 1		Peak 2			Peak 1		Peak 2
Ret.Time [min]		Ret.Time [min]			Ret.Time [min]		Ret.Time [min]
K-Value		K-Value			K-Value		K-Value
Plates/Column		Plates/Column			Plates/Column		Plates/Column
Volume [µL]		Volume [µL]			Volume [µL]		Volume [µL]
Injection Volume	Loading		Concentration		Injection Volume	Loading		Concentration
µL	µg	ng	µg/mL		µL	µg	ng	µg/mL
Flow cell		Selectivity α			Flow cell		Selectivity α
Path length [mm]		Resolution			Path length [mm]		Resolution
Volume [µL]		Sensitivity	1,00		Volume [µL]		Sensitivity
					Sensivity	Resolution	Pressure	Time
Gradient
1st Linear Down-Scaling
With same flow rate the gradient in the relationship of the column lengths is shortened and/or extended
Gradient Column 1					Gradient Column 2		Pressure
Flow Rate (mL/min)					Flow Rate (mL/min)		bar	psi
Time (min)	% B				Time (min)	% B
2nd Constant Gradient Volume
Independently of the column length the same eluent volume is always rinsed through the column
Gradient Column 1					Gradient Column 2		Pressure
Flow Rate (mL/min)					Flow Rate (mL/min)		bar	psi
Zeit (min)	% B				Zeit (min)	% B
	Vers.:4.6	© Jürgen Maier-Rosenkranz					info@jmr-analytik.de