JMR uHPLC Simulator
Info and Instructions
- the white cells are for input, the grey cells are calculating fields
- use '.' instead of ',' ; 'tab' instead of 'enter'
- this is a simulation sheet to help to choose the right column dimension and conditions
- the real values can differ from the calculated one
- the plate numbers are calculated with a reduced plate hight of 3dp
- the influence of the flow rate to plate numbers is not included - for particles <= 3 µm and an increased flow it doesn't influence the resolution to much
- the influence of the flow cell is high - therefore it is included in the calculation
- to realize the calculated values it is necessary to optimize injection volume, capillaries, rise time and data sampling rate
